Dr Siphathele Sibanda



Postdoctoral Fellow

Department

Biochemistry, Genetics and Microbiology
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Pantoea ananatis LMG 2665T synthesizes and utilizes acyl homoserine lactones (AHLs) for signaling. In my previous study, RNA-sequencing (RNA-seq) was used to identify the EanI/R quorum sensing regulon (complete set of genes regulated) in LMG 2665T. Using the transcriptome data, in my Postdoctoral research, I have identified a total of 75 small RNAs (sRNAs) expressed by the quorum sensing mutant and the wild type strain under the tested conditions. Quorum sensing regulated 8 out of the 75 expressed sRNAs.  I am currently working on validation of the RNA seq data using qRT PCR where differential expression of the sRNAs will be compared between the two strains, the quorum sensing mutant and the wild type.  To confirm the expression of sRNAs, PCR using cDNA as template will be done followed by sequencing.

 

 

 

 

 

 

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Book Chapters
Siphathele Sibanda, Lucy Moleleki, Divine Shyntum, Teresa Coutinho. 2018. Quorum Sensing in Gram-Negative Plant Pathogenic Bacteria. In: Advances in Plant Pathology. Intechopen., London SE1 9SG, United Kingdom, pp 67-89.
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Publication
Siphathele Sibanda, Stanford Kwenda, Collins K. Tanui, Divine Shyntum, Teresa Coutinho, Lucy Moleleki. (2018) Transcriptome profiling reveals the EanI/R quorum sensing regulon in Pantoea ananatis LMG 2665T. Genes 9(3):148. 10.3390/genes9030148
Sibanda S, Theron J, Shyntum DY, Moleleki LN, Coutinho TA. (2016) Characterization of two LuxI/R homologs in Pantoea ananatis LMG 2665 T. Canadian Journal of Microbiology 62(11):893-903. 10.1139/cjm-2016-0143