Ms Desre Pinard



PhD student

Department

Biochemistry, Genetics and Microbiology
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Primary Supervisor
Eshchar Mizrachi

Co Supervisor
Zander Myburg

 

In my PhD, I am studying how organelles impact wood formation in Eucalyptus. Although chloroplasts and mitochondria have been well studied, how these organelles and their genomes are integrated with the cell during xylogenesis is unknown. We have found that they form an integral hub for carbon metabolism during xylogenesis, and that plastids in tissues producing aromatic amino acids on a large scale may be a distinct type of plastid, which we call the “xyloplast”. In order to better understand the coordination of these genomes in wood formation, I have assembled and annotated high quality plastid and mitochondrial genomes for Eucalyptus grandis. These have been used to profile the complex transcriptomes of these organelles in leaf, xylem and phloem, and we have found that plastid transcriptomes in wood are unique compared to others that have been previously described from roots, fruits, and flowers in other plant species. In the future, I aim to fully characterize the plastids in wood forming tissues, including the circadian regulation of plastid metabolism, and the structure and function of xyloplasts for the production of monolignol precursors.

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Publication
Pinard D, Myburg AA, Mizrachi E. (2019) The plastid and mitochondrial genomes of Eucalyptus grandis. BMC Genomics 20(132):1-14. 10.1186/s12864-019-5444-4 PDF
Pinard D, Fierro AC, Marchal K, Myburg AA, Mizrachi E. (2019) Organellar carbon metabolism is co-ordinated with distinct developmental phases of secondary xylem. New Phytologist 10.1111/nph.15739
Pinard D, Mizrachi E. (2018) Unsung and understudied: plastids involved in secondary growth. Current Opinion in Plant Biology 42:30-36. 10.1016/j.pbi.2018.01.011
Pinard DS, Mizrachi E, Hefer C, Kersting AR, Joubert F, Douglas C, Mansfield SD, Myburg AA. (2015) Comparative analysis of plant carbohydrate active enZymes and their role in xylogenesis. BMC Genomics 16:402. 10.1186/s12864-015-1571-8 PDF
Myburg AA, Grattapaglia D, Tuskan GA, Hellsten U, Hayes RD, Grimwood J, Jenkins J, Lindquist E, Tice H, Bauer D, Goodstein DM, Dubchak I, Poliakov A, Mizrachi E, Kullan ARK, Hussey SG, Pinard D, Van der Merwe K, Singh P, et al. (2014) The genome of Eucalyptus grandis. Nature 510:356-362. 10.1038/nature13308
Botha J, Pinard D, Creux N, Hussey S, Maritz-Olivier C, Spokevicius A, Bossinger G, Mizrachi E, Myburg AA. (2011) Characterising the role of the Eucalyptus grandis SND2 promoter in secondary cell wall biosynthesis. BMC Proceedings 5(7):P105. 10.1186/1753-6561-5-S7-P105