High-throughput DNA Fingerprinting and Genotyping Research Service
The DNA Marker Ananlysis Facility is based in the Forest Molecular Genetics Laboratory at the University of Pretoria, and is supported by a team of technicians and geneticists. The facility offers high-throughput plant DNA isolation, fingerprinting and genotyping research services focused on commercially grown tree species and occassionally other plant species on request.
The following research capacity is available:
- DNA isolation from a range of plant tissues including leaves, pine needles, xylem scrapings, wood and bark cambium cores
- Microsatellite DNA fingerprinting for most Eucalyptus and Pinus species grown in South Africa, using 10-20 microsatellite markers
- Individual identification, clonal verification and parentage analysis for both Eucalyptus and pine species
- Species discrimination for selected Eucalyptus and Pinus species
The QuantStudio™ 12K Flex Real-time PCR System
The QuantStudio™ 12K Flex system is a comprehensive real-time PCR-based platform for the fast, accurate and affordable investigation, validation and screening of hundreds of target genes and samples. The QuantStudio™ system is highly flexible and includes five interchangeable blocks that can accommodate OpenArray plates, TaqMan array cards, 384-well plates which enable a researcher to scale up with the needs of individual experiments or projects.
The OpenArray system combined with the optimized pre-designed or custom TaqMan Assays is able to expand the number of samples and targets for maximum throughput analysis with its capacity to produce more than 12,000 high-quality, TaqMan data points per run. The streamlined workflow and minimal hands-on time of OpenArray technology enables the efficient analysis of a number of applications including:
- Large-scale gene expression analysis
- SNP genotyping
- microRNA analysis
- digital PCR
The QuantStudio™ 12K Flex instrument can be found in room 6-28 in the Natural and Agricultural Sciences Building at the University of Pretoria. Funding for the instrument was obtained from the NRF Equipment Platform (NEP) Grant. The instrument is thus a platform supporting genotyping and functional genomics projects on a national level. THe use of hte instrument is available to researchers in South Africa.