The Molecular Plant Physiology group was established in 1998 when Professor Karl Kunert joined FABI. Since then the group has grown and is currently headed by Dr Juan Vorster. We collaborate with various groups from around the world, each brining unique specialities, to understand plant development and the response of plant towards stress. Using a multi-disciplinary approach we hope to obtain a holistic view of the changes in the plant under stress. On a genomic level we have studies stress induced mutations and rearrangements of the genome, we are also using transcriptomics to study and identify genes and pathways that are important during the stress response. On a protein level we are especially interested in the role cysteine proteases as well as cysteine protease inhibitors play during development and senescence. Here we make use of in silico protein modelling to study protein-protein interactions as well as to predict the effect of mutations on inhibitor binding. Through protein engineering and enzyme kinetics we then evaluate the effect of these changes on inhibitor strength and specificity. We also measure protease activity and changes in the REDOX state in different tissues and under different stress conditions. To complement the molecular data we also follow and characterize various physiological and phenotypic changes in the plants we study. The ultimate aim is to identify usable molecular, biochemical or phenotypic markers that can be used to develop plants better adapted to stress in a changing environment.
Molecular Plant Physiology
Cullis C, Lawler DW, Chimwamurombe P, Kunert K, Bbebe N, Vorster J. (2019) Development of marama bean, an orphan legume, as a crop. Food and Energy Security e00164:1-12. 10.1002/fes3.164
NM Okumu, BJ Vorster, CF Reinhardt. (2019) Growth-stage and temperature influence glyphosate resistance in Conyza bonariensis (L.) Cronquist.. South African Journal of Botany 121:248-256. 10.1016/j.sajb.2018.10.034
Cristopher Cullis, Percy Chimwamurombe, Nigel Barker, Karl Kunert, Juan Vorster. (2018) Orphan Legumes Growing in Dry Environments: Marama Bean as a Case Study. Frontiers in Plant Science 9:11999. 10.3389/fpls.2018.01199
Magdeleen Cilliers, Stefan van Wyk, Phillipus van Heerden, Karl Kunert, Juan Vorster. (2018) Identification and changes of the drought-induced cysteine protease transcriptome in soybean (Glycine max) root nodules. Journal of Experimental and Environmental Botany 148:59-69. 10.1016/j.envexpbot.2017.12.005
Priyen Pillay, Karl Kunert, Eugene Makgopa, Chris Cullis, Juan Vorste. (2016) Agroinfiltration contributes to VP1 recombinant protein degradation. Bioengineered 7(6):459-477. 10.1080/21655979.2016.1208868